Granulocyte-macrophage colony-stimulating factor (GM-CSF) is an essential cytokine that regulates the production, differentiation, and activity of granulocytes and macrophages, key cells of the immune system. Recombinant human GM-CSF (rhGM-CSF) is a synthetic form of this cytokine, produced by recombinant DNA technology and widely used in both research and clinical settings.
Product Overview : Human Recombinant GM-CSF 300 µg
Human Recombinant GM-CSF 300 µg is a highly pure and bioactive cytokine designed for multiple applications. This lyophilized protein is composed of 128 amino acids, with a molecular weight of approximately 14.6 kDa. It is produced using Escherichia coli (E. coli) expression systems, which ensure high efficiency and scalability of protein production.
Key Features and Specifications
- Purity and Activity : Recombinant GM-CSF has a purity greater than 98%, ensuring minimal contaminants. Its biological activity is demonstrated by the ability to stimulate the proliferation of human TF-1 cells, with an effective dose (ED50) ≤ 0.1 ng/ml, corresponding to a specific activity ≥ 1 x 10⁷ units/mg.
- Endotoxin Levels : The endotoxin concentration is less than 1 EU/µg, making the product suitable even for sensitive experimental applications.
Applications in Research and Medicine
Recombinant human GM-CSF has multiple applications, including:
- Hematopoietic Research : It is fundamental in the studies on hematopoiesis, or the process of formation of blood cells, allowing to deepen the knowledge on the functioning and disorders of the bone marrow.
- Immunology : Used to investigate immune responses, particularly the activation and differentiation of dendritic cells and macrophages.
- Clinical Therapies : Clinically, GM-CSF is used to accelerate the recovery of white blood cells after chemotherapy, promoting a more rapid reconstitution of the immune system. It also finds promising application in the development of vaccines as an adjuvant, to enhance immune responses.
Product Storage and Handling
To ensure optimal stability and activity, it is recommended to store lyophilized recombinant GM-CSF at -20°C. After reconstitution, the protein should be stored between 2-8°C and used within one week to maintain maximum potency.
The product GM-CSF Recombinant Human 300 µg is an essential tool for both research and clinical application, offering high purity and biological activity. Its applications in the understanding of immune mechanisms and the development of therapeutic strategies highlight its importance for the advancement of medical science
